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chameleon ii 2 photon laser  (Coherent Corp)


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    Coherent Corp chameleon ii 2 photon laser
    Chameleon Ii 2 Photon Laser, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 99/100, based on 9617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/photon+chameleon+laser/Chameleon/bio_rxiv__64898__2026__03__11__710817-153-12-16
    Average 99 stars, based on 9617 article reviews
    chameleon ii 2 photon laser - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: A fluorescence lifetime-based FLIM-timer for measuring the protein turnover of transcription factor Nrf2 in live cells
    Article Snippet: .. Fluorescence lifetime was measured using SPC-150 Time-Correlated Single Photon Counter (TCSPC) module (Becker&Hickl) attached to LSM 710 microscope (Zeiss) equipped with either InTune pulsed laser with repetition rate 40 MHz and tunable wavelength (Zeiss) or 2-photon Chameleon laser with pulse repetition rate 80 MHz (Coherent) and HPM-100-40GaAsP detector that feeds to TCSPC module. .. FLIM data acquisition is operated by SPCM software (Becker&Hickl) controlling TCSPC module. sfGFP fluorescence lifetime was measured at 490 nm (InTune laser) or 920 nm (2-photon laser) wavelength and the 500–550 nm bandpass filters suitable for GFP.

    Article Title: Design and Validation of Flim-timer for Measuring Protein Turnover in Cells Using Fluorescence Lifetime
    Article Snippet: .. Fluorescence lifetime was measured using SPC-150 Time-Correlated Single Photon Counter (TCSPC) module (Becker&Hickl) attached to LSM 710 microscope (Zeiss) equipped with either InTune pulsed laser with repetition rate 40 MHz and tunable wavelength (Zeiss) or 2- photon Chameleon laser with pulse repetition rate 80 MHz (Coherent) and HPM-10040GaAsP detector that feeds to TCSPC module. .. FLIM data acquisition is operated by SPCM software (Becker&Hickl) controlling TCSPC module. sfGFP fluorescence lifetime was measured at 490 nm (InTune laser) or 920 nm (2-photon laser) wavelength and the filters suitable for GFP.

    Microscopy:

    Article Title: A fluorescence lifetime-based FLIM-timer for measuring the protein turnover of transcription factor Nrf2 in live cells
    Article Snippet: .. Fluorescence lifetime was measured using SPC-150 Time-Correlated Single Photon Counter (TCSPC) module (Becker&Hickl) attached to LSM 710 microscope (Zeiss) equipped with either InTune pulsed laser with repetition rate 40 MHz and tunable wavelength (Zeiss) or 2-photon Chameleon laser with pulse repetition rate 80 MHz (Coherent) and HPM-100-40GaAsP detector that feeds to TCSPC module. .. FLIM data acquisition is operated by SPCM software (Becker&Hickl) controlling TCSPC module. sfGFP fluorescence lifetime was measured at 490 nm (InTune laser) or 920 nm (2-photon laser) wavelength and the 500–550 nm bandpass filters suitable for GFP.

    Article Title: Design and Validation of Flim-timer for Measuring Protein Turnover in Cells Using Fluorescence Lifetime
    Article Snippet: .. Fluorescence lifetime was measured using SPC-150 Time-Correlated Single Photon Counter (TCSPC) module (Becker&Hickl) attached to LSM 710 microscope (Zeiss) equipped with either InTune pulsed laser with repetition rate 40 MHz and tunable wavelength (Zeiss) or 2- photon Chameleon laser with pulse repetition rate 80 MHz (Coherent) and HPM-10040GaAsP detector that feeds to TCSPC module. .. FLIM data acquisition is operated by SPCM software (Becker&Hickl) controlling TCSPC module. sfGFP fluorescence lifetime was measured at 490 nm (InTune laser) or 920 nm (2-photon laser) wavelength and the filters suitable for GFP.



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    ( A ) Experimental timeline. LID mice were euthanized 24 to 48 hours or 30 min after the last levodopa injection for ex vivo assessment of the off- and on-state, respectively. ( B ) Confocal image of a coronal brain slice from a unilaterally 6-OHDA–lesioned, LID on-state mouse immunostained with anti-pERK (red) and anti–tyrosine hydroxylase (TH; green) antibodies. Scale bar, 0.5 mm. ( C ) Schematic illustrating the somatic excitability assay in dSPNs. ( D ) Sample voltage recordings from dSPNs in the off- and on-state of LID in response to a 200-pA current injection. Scale bars, 10 mV and 200 ms. ( E ) Current-response curves showing that somatic excitability of dSPNs was state dependent (control: n = 10 cells from six mice; 6-OHDA: n = 12 cells from 5 mice; off-state: n = 15 cells from 8 mice; on-state: n = 15 cells from 10 mice; on-state + SCH: n = 10 cells from 5 mice). ( F ) Box plot summary of rheobase in dSPNs. **** P < 0.0001; Mann-Whitney test. ctr, control. ( G <t>)</t> <t>Two-photon</t> laser scanning microscopy (2PLSM) image of a patched dSPN with imaging sites on proximal and distal dendrites indicated. Scale bar, 20 μm. ( H ) Current injections of 2 nA (top) are aligned with evoked somatic spikes (middle) and fluorescence transients recorded from proximal and distal dendrites (bottom). Scale bars, 25 mV, 0.1 ΔG/R 0 , and 0.5 s. ( I ) Box plot summary of dendritic excitability index [the distal-to-proximal ratio of area under the curve (AUC) of ΔG/R 0 ] of dSPNs (control: n = 11 cells from 6 mice; 6-OHDA: n = 9 cells from 5 mice; off-state: n = 10 cells from 7 mice; on-state: n = 10 cells from 5 mice; on-state + SCH: n = 9 cells from 5 mice). * P < 0.05; Mann-Whitney test.
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    ( A ) Experimental timeline. LID mice were euthanized 24 to 48 hours or 30 min after the last levodopa injection for ex vivo assessment of the off- and on-state, respectively. ( B ) Confocal image of a coronal brain slice from a unilaterally 6-OHDA–lesioned, LID on-state mouse immunostained with anti-pERK (red) and anti–tyrosine hydroxylase (TH; green) antibodies. Scale bar, 0.5 mm. ( C ) Schematic illustrating the somatic excitability assay in dSPNs. ( D ) Sample voltage recordings from dSPNs in the off- and on-state of LID in response to a 200-pA current injection. Scale bars, 10 mV and 200 ms. ( E ) Current-response curves showing that somatic excitability of dSPNs was state dependent (control: n = 10 cells from six mice; 6-OHDA: n = 12 cells from 5 mice; off-state: n = 15 cells from 8 mice; on-state: n = 15 cells from 10 mice; on-state + SCH: n = 10 cells from 5 mice). ( F ) Box plot summary of rheobase in dSPNs. **** P < 0.0001; Mann-Whitney test. ctr, control. ( G <t>)</t> <t>Two-photon</t> laser scanning microscopy (2PLSM) image of a patched dSPN with imaging sites on proximal and distal dendrites indicated. Scale bar, 20 μm. ( H ) Current injections of 2 nA (top) are aligned with evoked somatic spikes (middle) and fluorescence transients recorded from proximal and distal dendrites (bottom). Scale bars, 25 mV, 0.1 ΔG/R 0 , and 0.5 s. ( I ) Box plot summary of dendritic excitability index [the distal-to-proximal ratio of area under the curve (AUC) of ΔG/R 0 ] of dSPNs (control: n = 11 cells from 6 mice; 6-OHDA: n = 9 cells from 5 mice; off-state: n = 10 cells from 7 mice; on-state: n = 10 cells from 5 mice; on-state + SCH: n = 9 cells from 5 mice). * P < 0.05; Mann-Whitney test.
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    Image Search Results


    ( A ) Experimental timeline. LID mice were euthanized 24 to 48 hours or 30 min after the last levodopa injection for ex vivo assessment of the off- and on-state, respectively. ( B ) Confocal image of a coronal brain slice from a unilaterally 6-OHDA–lesioned, LID on-state mouse immunostained with anti-pERK (red) and anti–tyrosine hydroxylase (TH; green) antibodies. Scale bar, 0.5 mm. ( C ) Schematic illustrating the somatic excitability assay in dSPNs. ( D ) Sample voltage recordings from dSPNs in the off- and on-state of LID in response to a 200-pA current injection. Scale bars, 10 mV and 200 ms. ( E ) Current-response curves showing that somatic excitability of dSPNs was state dependent (control: n = 10 cells from six mice; 6-OHDA: n = 12 cells from 5 mice; off-state: n = 15 cells from 8 mice; on-state: n = 15 cells from 10 mice; on-state + SCH: n = 10 cells from 5 mice). ( F ) Box plot summary of rheobase in dSPNs. **** P < 0.0001; Mann-Whitney test. ctr, control. ( G ) Two-photon laser scanning microscopy (2PLSM) image of a patched dSPN with imaging sites on proximal and distal dendrites indicated. Scale bar, 20 μm. ( H ) Current injections of 2 nA (top) are aligned with evoked somatic spikes (middle) and fluorescence transients recorded from proximal and distal dendrites (bottom). Scale bars, 25 mV, 0.1 ΔG/R 0 , and 0.5 s. ( I ) Box plot summary of dendritic excitability index [the distal-to-proximal ratio of area under the curve (AUC) of ΔG/R 0 ] of dSPNs (control: n = 11 cells from 6 mice; 6-OHDA: n = 9 cells from 5 mice; off-state: n = 10 cells from 7 mice; on-state: n = 10 cells from 5 mice; on-state + SCH: n = 9 cells from 5 mice). * P < 0.05; Mann-Whitney test.

    Journal: Science Advances

    Article Title: State-dependent modulation of spiny projection neurons controls levodopa-induced dyskinesia in a mouse model of Parkinson’s disease

    doi: 10.1126/sciadv.adv8224

    Figure Lengend Snippet: ( A ) Experimental timeline. LID mice were euthanized 24 to 48 hours or 30 min after the last levodopa injection for ex vivo assessment of the off- and on-state, respectively. ( B ) Confocal image of a coronal brain slice from a unilaterally 6-OHDA–lesioned, LID on-state mouse immunostained with anti-pERK (red) and anti–tyrosine hydroxylase (TH; green) antibodies. Scale bar, 0.5 mm. ( C ) Schematic illustrating the somatic excitability assay in dSPNs. ( D ) Sample voltage recordings from dSPNs in the off- and on-state of LID in response to a 200-pA current injection. Scale bars, 10 mV and 200 ms. ( E ) Current-response curves showing that somatic excitability of dSPNs was state dependent (control: n = 10 cells from six mice; 6-OHDA: n = 12 cells from 5 mice; off-state: n = 15 cells from 8 mice; on-state: n = 15 cells from 10 mice; on-state + SCH: n = 10 cells from 5 mice). ( F ) Box plot summary of rheobase in dSPNs. **** P < 0.0001; Mann-Whitney test. ctr, control. ( G ) Two-photon laser scanning microscopy (2PLSM) image of a patched dSPN with imaging sites on proximal and distal dendrites indicated. Scale bar, 20 μm. ( H ) Current injections of 2 nA (top) are aligned with evoked somatic spikes (middle) and fluorescence transients recorded from proximal and distal dendrites (bottom). Scale bars, 25 mV, 0.1 ΔG/R 0 , and 0.5 s. ( I ) Box plot summary of dendritic excitability index [the distal-to-proximal ratio of area under the curve (AUC) of ΔG/R 0 ] of dSPNs (control: n = 11 cells from 6 mice; 6-OHDA: n = 9 cells from 5 mice; off-state: n = 10 cells from 7 mice; on-state: n = 10 cells from 5 mice; on-state + SCH: n = 9 cells from 5 mice). * P < 0.05; Mann-Whitney test.

    Article Snippet: A two-photon laser (Chameleon Ultra II, Coherent, Santa Clara, CA) tuned to 920 nm was used to excite GRAB ACh3.0 .

    Techniques: Injection, Ex Vivo, Slice Preparation, Control, MANN-WHITNEY, Laser-Scanning Microscopy, Imaging, Fluorescence